Biomolecules & Therapeutics 2008; 16(2): 95-99  
Fumonisin B1 Induces Apoptosis in Sphingosine 1-Phosphate Lyase-null F9 Cells through Increase of Sphingolipids Levels
Seon-Mi PAK1, Nam-Young PARK1, Myung-Yong PARK1, Wan-Jong KIM2, Jong-Hwa LEE2, Seikwan OH3, Hwan-Soo YOO1, and Yong-Moon LEE1*
1College of Pharmacy, Chungbuk National University, 21 Kaesin-Dong, Chongju 361-763, Korea
2Department of Life Sciences, Soonchunhyang University, Asan 336-745, Korea
3Department of Neuroscience, School of Medicine, Ewha Womans University, Seoul 158-710, Korea
Received: May 27, 2008; Accepted: June 6, 2008; Published online: June 1, 2008.
© The Korean Society of Applied Pharmacology. All rights reserved.

Abstract
Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1- phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)- induced apoptosis, suggesting differential apoptotic processes.
Keywords: Apoptosis, Sphingosine 1-phosphate, Release, F9 cells, HPLC

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