Biomolecules & Therapeutics 2008; 16(2): 82-86  
Effects of the Constituents of Gardenia Fructus on Prostaglandin and NO Production
Hyun LIM1, Kwang-Rock PARK2, Dong-Ung LEE2, Yeong Shik KIM3, and Hyun Pyo KIM1*
1College of Pharmacy, Kangwon National University, Chunchon 200-701, Korea
2Dept. Biotechnology, Dongguk University, Gyeongju 780-714, Korea
2Natural Products Research Institute and College of Pharmacy, Seoul National University, Seoul 110-460, Korea
Received: February 11, 2008; Accepted: April 3, 2008; Published online: June 30, 2008.
© The Korean Society of Applied Pharmacology. All rights reserved.

The fruits of Gardenia jasminoides Ellis have been previously reported to possess anti-inflammatory activity. In this study, the constituents including geniposide, geniposidic acid, genipin and crocin were evaluated for their effects on prostaglandin and NO production in an attempt to establish anti-inflammatory cellular mechanisms. Among the constituents tested, only genipin significantly inhibited cyclooxygenase-2-mediated PGE2 and inducible nitric oxide synthase-mediated NO production from lipopolysaccharide-treated RAW 264.7 cells at 10 -100 μM. Genipin also inhibited nuclear transcription factor-κB activation. Moreover, genipin showed in vivo antiinflammatory activity on λ-carrageenan-induced paw edema in mice (10.4-29.9% inhibition at 20-100 mg/kg, i.p.). All of these results suggest that genipin may contribute to anti-inflammatory activity of the fruits of G. jasminoides and an inhibitory action on prostaglandin and NO production is, at least, the part of anti-inflammatory mechanism of genipin.
Keywords: Gardenia jasminoides Ellis, genipin, cyclooxygenase, nitric oxide synthase, anti-inflammation

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