Biomolecules & Therapeutics  https://doi.org/10.4062/biomolther.2022.084
Functional Characterization of Drosophila melanogaster CYP6A8 Fatty Acid Hydroxylase
Sang-A Lee1, Vitchan Kim1, Byoungyun Choi1, Hyein Lee2, Young-Jin Chun2, Kyoung Sang Cho1 and Donghak Kim1,*
1Department of Biological Sciences, Konkuk University, Seoul 05025,
2College of Pharmacy, Chung Ang University, Seoul 06974, Republic of Korea
*E-mail: donghak@konkuk.ac.kr
Tel: +82-2-450-3366, Fax: +82-2-3436-5432
Received: June 20, 2022; Revised: July 15, 2022; Accepted: July 20, 2022; Published online: August 8, 2022.
© The Korean Society of Applied Pharmacology. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Genomic analysis indicated that the genome of Drosophila melanogaster contains more than 80 cytochrome P450 genes. To date, the enzymatic activity of these P450s has not been extensively studied. Here, the biochemical properties of CYP6A8 were characterized. CYP6A8 was cloned into the pCW vector, and its recombinant enzyme was expressed in Escherichia coli and purified using Ni2+-nitrilotriacetate affinity chromatography. Its expression level was approximately 130 nmol per liter of culture. Purified CYP6A8 exhibited a low-spin state in the absolute spectra of the ferric forms. Binding titration analysis indicated that lauric acid and capric acid produced type І spectral changes, with Kd values 28 ± 4 and 144 ± 20 μM, respectively. Ultra-performance liquid chromatography–mass spectrometry analysis showed that the oxidation reaction of lauric acid produced (ω-1)-hydroxylated lauric acid as a major product and ω-hydroxy-lauric acid as a minor product. Steady-state kinetic analysis of lauric acid hydroxylation yielded a kcat value of 0.038 ± 0.002 min–1 and a Km value of 10 ± 2 μM. In addition, capric acid hydroxylation of CYP6A8 yielded kinetic parameters with a kcat value of 0.135 ± 0.007 min–1 and a Km value of 21 ± 4 μM. Because of the importance of various lipids as carbon sources, the metabolic analysis of fatty acids using CYP6A8 in this study can provide an understanding of the biochemical roles of P450 enzymes in many insects, including Drosophila melanogaster.
Keywords: P450, CYP6A8, Drosophila melanogaster, Lauric acid, Capric acid, Mass spectrometry


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