Biomolecules & Therapeutics 2022; 30(4): 328-333  https://doi.org/10.4062/biomolther.2022.014
Repeated Morphine Administration Increases TRPV1 mRNA Expression and Autoradiographic Binding at Supraspinal Sites in the Pain Pathway
Thi-Lien Nguyen1,2, Yun-Son Nam1, Seok-Yong Lee1 and Choon-Gon Jang1,*
1Department of Pharmacology, School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea
2Pharmacology Laboratory, National Institute of Drug Quality Control, Ha Noi 100000, Viet Nam
*E-mail: jang@skku.edu
Tel: +82-31-290-7780, Fax: +82-31-292-8800
Received: January 26, 2022; Revised: April 18, 2022; Accepted: April 29, 2022; Published online: May 26, 2022.
© The Korean Society of Applied Pharmacology. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Repeated morphine administration induces tolerance to its analgesic effects. A previous study reported that repeated morphine treatment activates transient receptor potential vanilloid type 1 (TRPV1) expression in the sciatic nerve, dorsal root ganglion, and spinal cord, contributing to morphine tolerance. In the present study, we analyzed TRPV1 expression and binding sites in supraspinal pain pathways in morphine-tolerant mice. The TRPV1 mRNA levels and binding sites were remarkably increased in the cortex and thalamus of these animals. Our data provide additional insights into the effects of morphine on TRPV1 in the brain and suggest that changes in the expression of, and binding to TRPV1 in the brain are involved in morphine tolerance.
Keywords: TRPV1, Morphine, RT-PCR, RTX, Autoradiography, Tolerance


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