Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 5. PA increases the activities of caspase 7, 8 and 9 in MCF-7 cells. (A) PA increased the protein expression of caspase-7 in a dose-dependent manner (10, 15 and 20 μM of PA). The results were expressed as free fluorescent of DEVD-AFC. (B) PA increased the activity of caspase-8 at the highest dose of PA (20 μM). (C) PA increased the activity of caspase-9 at the highest dose of PA (20 μM). The caspase-7, 8 and -9 activities were determined by incubation with the specific substrates Ac-DEVD-AFC (abbreviated as N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Trifluoromethyl-coumarin) (for caspase 7), Ac-IETD-pNA (abbreviated as Ac-Ile-Glu-Thr-Asp-pNA (pNA=p-Nitroaniline)) (for caspase 8) and Ac-LEHD-pNA (abbreviated as Ac-Leu-Glu-His-Asp-pNA (pNA=p-Nitroaniline)) (for caspase 9). The optical density was measured at 405 nm as described in the Materials and Methods section. The results are from 3 independent experiments and are presented as means ± SD. *Indicates a significant difference by Tukey’s test (p<0.05) relative to their respective control.
Biomolecules & Therapeutics 2018;26:328~334
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