Fig. 5. The inhibitory effects of compound 2, 4, 5 on TNF-α, IL-1β and IL-6. Cardiomyocytes were exposed to CVB3 for 1 h, washed with PBS three times, and incubated with compound 2, 4, 5, or cinnamaldehyde (10 μM). After 72-hours of culturing, the supernatants were collected, and the productions of TNF-α, IL-1β and IL-6 were detected with ELISA (n=6) (A). The TNF-α, IL-1β and IL-6 mRNA expression in cardiomyocytes were assayed using real-time PCR after culturing for 12 h (B). (C) Western blot depicting TNF-α, IL-1β and IL-6 expression in the cardiomyocytes. Typical results are depicted in left panel along with the statistical analysis of TNF-α, IL-1β or IL-6 to GAPDH expression in right panel (n=3). Values are expressed as mean ± SD. Significant differences between two groups were determined by one-way ANOVA. *p<0.05 vs. CVB3 control.
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