Fig. 4. Effects of EUE on LPS-induced ROS accumulation in BV-2 microglial cells. (A) Cells were pretreated with the indicated concentrations of EUE for 30 min and then stimulated with 100 ng/ml LPS for 24 h. Intracellular ROS accumulation was assayed using DCFH-DA fluorescent dye. Data are presented as means ± S.E.M. (n=6). Nuclei were counterstained with Hoechst 33258, and representative pictures were taken with a fluorescence microscope (B, 20× magnification). The images shown are representative of three experiments. ***p<0.001 compared with the control group. ###p<0.001 compared with the LPS-treated group. Scale bar: 200 μm.
© Biomolecules & Therapeutics
