Fig. 2. Effects of EUE on LPS-mediated upregulation of the protein and mRNA levels of iNOS (A and C) and COX-2 (B and D) in BV-2 microglial cells. Cells were pretreated with the indicated concentrations of EUE for 30 min and then stimulated with 100 ng/ml LPS for 24 h (A and B). The levels of iNOS, COX-2, and β-actin were determined by Western blot analysis. Cells were pretreated with the indicated concentrations of EUE for 30 min and then stimulated with 100 ng/ml LPS for 6 h (C and D). The mRNA expression levels of iNOS, COX-2, and β-actin were determined by RT-PCR. Densitometric results are presented as means ± S.E.M. (n=3). ***p<0.001 compared with the control group. ##p<0.01 and ###p<0.001 compared with the LPS-treated group.
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