Fig. 1. Effects of EUE on the viability of BV-2 microglial cells (A). Cells were treated with the indicated concentrations of EUE for 24 h. Effects of EUE on LPS-induced cell death in BV-2 microglial cells (B). Cell viability was assessed by the MTT assay and is expressed as a percentage of the corresponding value of control cells. Cells were pretreated with the indicated concentrations of EUE for 30 min and then stimulated with 100 ng/ml LPS for 24 h. Effects of EUE on LPS-induced production of NO (C) and PGE2 (D) in BV-2 microglial cells. Cells were pretreated with the indicated concentrations of EUE for 30 min and then stimulated with 100 ng/ml LPS for 24 h. The concentrations of nitrite in the culture medium were then determined by the Griess reagent. The PGE2 concentrations in the culture medium were measured using a commercial ELISA kit. Data are presented as means ± S.E.M. (n=6). ***p<0.001 compared with the control group. #p<0.05 and ###p<0.001 compared with the LPS-treated group.
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