Fig. 3. Increased expression and activity of sphingosine-1-phosphate phosphatase in KC following Vit C. Human primary keratinocytes (KC) were cultured in high Ca2+-supplemented medium with or without Vit C, as described in materials and methods. Sphingosine-1-phosphate phosphatase (SPPase) mRNA expression was determined by qRT-PCR (A). Protein expression of SPPase protein was determined by western immunoblot analysis (B). SPPase activity was analyzed by ESI-LC/MS/MS (C). Data are means ± SD (n=3). *p<0.01 versus vehicle control.
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