Fig. 4. The effect of fisetin on the upstream signaling cascade of NF-κB. (A and B) RAW264.7 cells were incubated with LPS (1 μg/mL) in the presence or absence of fisetin (30 μM) for 30 min. The total phospho-protein levels of Src, Syk, and IκBα from whole lysates were determined by immunoblotting analysis.
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