Fig. 3. The effect of fisetin on transcriptional activation in LPS-stimulated RAW264.7 cells. (A and B) The mRNA levels of iNOS, COX-2, and TNF-α expressed in LPS (1 μg/mL)-treated RAW264.7 cells in the presence or absence of fisetin (20 and 30 μM) were measured by RT-PCR (A) or real-time PCR (B). (C) The nuclear levels of p65 and p50 in RAW264.7 cells treated with LPS (1 μg/mL) in the presence or absence of fisetin (30 VM) were analyzed by immunoblotting analysis. Data (B) are expressed as the mean ± SD of experiments, which were performed with six samples. **p<0.01 compared to normal or control groups.
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