Fig. 4. Detection of oxidized DNA bases, namely (A) 8-hydroxy-2′-deoxyguanosine (8-OHdG) and (B) N6-etheno-2-deoxyadenosine after treatment of HaCaT keratinocytes with the kINPen 09 or hydrogen peroxide (100 μM). 24 h after exposure of cells to plasma they were fixed and stained with the antibodies 2E2 for 8-OHdG for and EMA-1 for N6-etheno-2′-desoxyadenosine. Binding of antibodies was detected by using flow cytometry. Mean fluorescence intensities (MFI) are expressed as percentage of that of untreated control cells. Mean ± SEM, *p<0.05 vs. untreated control cells.
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