Fig. 6. Effects of CE-WIB801C on collagen-induced TXA2 production, and TXA2-associated enzymes (COX-1 and TXAS) activities. (A) Effects of CE-WIB801C on TXB2 production. Washed platelets (108/ml) were preincubated with or without CE-WIB801C in the presence of 2 mM CaCl2 for 3 min at 37°C, then stimulated with collagen (10 μg/ml) for 5 min in an aggregometer. TXA2 was determined with the content of TXB2 using TXB2 EIA kit as described in “Materials and Methods”. (B) Effects of CE-WIB801C on COX-1 activity in platelet lysates. (C) Effects of CE-WIB801C on TXAS activity in platelet lysates. COX-1 and TXAS activity was determined with COX fluorescent activity assay and TXB2 EIA kit as described in “Materials and Methods”. The data are expressed as the mean ± S.E.M. (n=4). ap<0.05 versus non-stimulated platelets, **p<0.001 versus the collagen-stimulated platelets.
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