Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 4. Effects of CE-WIB801C on collagen-induced [Ca2+]i mobilization, and in the presence of A-kinase inhibitor (Rp-8-Br-cAMPS) or G-kinase inhibitor (Rp-8-Br-cGMPS). (A) Effect of CE-WIB801C on collagen-induced [Ca2+]i mobilization. (B) Effect of CE-WIB801C on collagen-induced [Ca2+]i mobilization in the presence of A-kinase inhibitor (Rp-8-Br-cAMPS). (C) Effect of CE-WIB801C on collagen-induced [Ca2+]i mobilization in the presence of G-kinase inhibitor (Rp-8-Br-cGMPS). Fura 2-loaded washed platelets (108/ml) were preincubated with or without CE-WIB801C, the A-kinase inhibitor Rp-8-Br-cAMPS or the G-kinase inhibitor Rp-8-Br-cGMPS in the presence of 2 mM CaCl2 for 3 min at 37°C, and then collagen (10 μg/ml) was added. [Ca2+]i was determined as described in “Materials and Methods”. The data are expressed as the mean ± S.E.M. (n=4). ap<0.05 versus non-stimulated platelets, **p<0.001 versus the collagen-stimulated platelets, ††p<0.001 versus each control: the collagen-stimulated platelets in the presence of CE-WIB801C (400 μg/ml).
Biomol Ther (Seoul) 2014;22:223~231 https://doi.org/10.4062/biomolther.2014.025
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