Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 3. Effects of CE-WIB801C on collagen-induced platelet aggregation. (A) Effects of CE-WIB801C pretreatment on collagen-induced platelet aggregation. (B) IC50 value of CE-WIB801C on collagen-induced platelet aggregation. Washed platelets (108/ml) were preincubated with or without various concentrations of CE-WIB801C (25 to 400 μg/ml) in the presence of 2 mM CaCl2 for 3 min at 37°C, then stimulated with collagen (10 μg/ml) for 5 min in aggregometer. Platelet aggregation (%) was recorded as an increase in light transmission. Inhibition rate by CE-WIB801C was recorded as percentage of the collagen-induced aggregation rate. IC50 value of CE-WIB801C was calculated by 4-parameter log fit method. The data are expressed as the mean ± S.E.M. (n=4). *p<0.05, **p<0.001 versus the collagen-stimulated platelets.
Biomol Ther (Seoul) 2014;22:223~231
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