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Fig. 2. HPLC chromatograms of CE-WIB801C and authentic compounds (adenine, adenosine, and cordycepin). (A) The chromatogram of CE-WIB801C. (B) The chromatogram of authentic compounds (adenine, adenosine, and cordycepin). HPLC was performed on a Zorbax ODS C18 column (250 mm×4.6 mm id, 5 μm) and a Zorbax ODS C18 guard column (12.5 mm×4.6 mm id, 5 μm) were used at 25°C. The mobile phase consisted of water (a) and methanol with 0.01M KH2PO4 (b) using the following program: 0?30 min, 15% b. The flow rate was at 1.0 ml/min and sample injection volume was 10 μl. The UV detection was operated at 254 nm.
Biomol Ther (Seoul) 2014;22:223~231 https://doi.org/10.4062/biomolther.2014.025
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