Fig. 2. Effects of resveratrol on the expression of melanogenesis-related proteins in B16F10 cells. B16F10 cells were treated with 1, 10, and 100 μM resveratrol for 48 h. All groups except the normal group stimulated melanogenesis by α-MSH. Forty micrograms of total protein from the cell lysates were used for Western blot analysis (A). The graph of protein expression, tyrosinase (B), TRP-1 (C), TRP-2 (D) and MITF (E) were normalized to the band corresponding to α-tubulin. Student’s t-test was used for statistical analyses, *p<0.05 and **p<0.01 indicate statistically significant differences compared to the normal. #p<0.05, ##p<0.01 and ###p<0.001 indicate statistically significant differences compared to the control.
© Biomolecules & Therapeutics
