Fig. 6. Effect of emodin on the nuclear expression of Sp1 in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of emodin for 24 h and then stimulated with epidermal growth factor (EGF) (25 ng/mL) for 60 min. The nuclear protein extracts were prepared and subjected to western blot analysis using antibody against Sp1. As a loading control, p84 levels were analyzed. Three independent experiments were performed and the representative data were shown. *Significantly different from control (p<0.05). †Significantly different from EGF alone (p<0.05). cont: control, E: emodin, Sp1: specificity protein-1, concentration unit is μM.
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