Fig. 4. Effect of emodin on EGF-induced MUC5AC mucin glycoprotein production from NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of emodin for 30 min and then stimulated with EGF (25 ng/mL), for 24 h. Cell lysates were collected for measurement of MUC5AC mucin glycoprotein production by ELISA. Each bar represents a mean ± SEM of 3 culture wells compared to the control set at 100%. Three independent experiments were performed, and the representative data were shown. *Significantly different from control (p<0.05). †Significantly different from EGF alone (p<0.05). cont: control, E: emodin, concentration unit is μM.
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