Fig. 3. Effect of emodin on the phosphorylation of MEK1/2, the phosphorylation of p44/42, and the phosphorylation of p38, in NCI-H292 cells. NCI-H292 cells were pretreated with varying concentrations of emodin for 24 h and then stimulated with epidermal growth factor (EGF) (25 ng/mL) for 15 min. Whole cell extract was collected and western blot analysis of the cellular proteins with anti-phospho-MEK1/2 (Ser221) (A), anti-phospho-p44/42 (Thr202/Tyr204) (B), and anti-phospho-p38 (Thr180/Tyr182) (C) antibodies was conducted. Three independent experiments were performed and the representative data were shown. *Significantly different from control (p<0.05). †Significantly different from EGF alone (p<0.05). cont: control, E: emodin, concentration unit is μM.
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