Fig. 5. ISO inhibits CXCL12-induced invasion of colon cancer cells via inhibition of CXCR4 expression. (A, B) HCT116 cells were incubated with the indicated concentrations of ISO for 24 h or with 80 μM of ISO for indicated time. β-actin was used to confirm equal sample loading. (C, D) HCT116 cells were incubated with the indicated concentrations of ISO for 24 h or with 80 μM of ISO for indicated time. Total RNA was isolated and analyzed by real-time PCR assays. GAPDH was used to confirm equal sample loading. (E, F) HCT116 cells were incubated with the indicated concentrations of ISO for 24 h or with 80 μM of ISO for indicated time. The nuclear extracts were analyzed for NF-κB-DNA binding activity; bars give the standard error (*p<0.05, ***p<0.001 vs control).
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