Fig. 4. ISO inhibits CXCL12-induced invasion of MDA-MB-231 and HCT116 cells. (A) MDA-MB-231 cells were seeded on the top of matrigel chamber. After incubation with or without 80 μM of ISO for 24 h, transwell chambers were placed in 24-well plate, in which we added the basal medium only or basal medium containing 100 ng/mL CXCL12. After incubation, cell invasion ability was observed. (B) Columns give the mean numbers of invaded cells; bars give the standard error (*p<0.05, CON vs CXCL12; ###p<0.001 CXCL12 vs treated ISO group). (C) HCT116 cells were seeded on the top of matrigel chamber. After incubation with or without 80 μM of ISO for 24 h, transwell chambers were placed into the wells of a 24-well plate, in which we added either the basal medium only or basal medium containing 100 ng/mL CXCL12. After that, cell invasion ability was observed. (D) Columns give the mean numbers of invaded cells; bars give the standard error (***p<0.001 CON vs CXCL12; ###p<0.001 CXCL12 vs treated ISO group).
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