Fig. 2. ISO downregulates CXCR4 transcription, but not via its protein degradation. (A, B) MDA-MB-231 cells were treated with indicated concentrations of lactacystin or chloroquine for 1 h at 37°C, followed by treatment with 80 μM of ISO for 24 h. Whole-cell extracts were analyzed by Western blot analysis using CXCR4 antibody. β-actin was used to confirm equal sample loading. (C, D) MDA-MB-231 cells were treated with ISO (20, 40, and 80 μM) for 24 h or with the 80 μM of ISO for the indicated time. Total RNA was isolated and analyzed by RT-PCR assays. GAPDH was used to confirm equal sample loading.
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