Fig. 3. Jolkinolide B inhibits PA-induced lipid accumulation and lipogenesis. (A) Primary hepatocyte lipid accumulation was presented by Oil Red O staining. Red-stained intracellular fat content was quantified with a fluorescence microscope. The images were shown at 20× magnifications for each experimental condition (scale bar=100 μm). (B) Primary hepatocyte lipid accumulation was presented by BODIPY staining. Lipid droplet-stained BODIPY was imaged with a fluorescence microscope. BODIPY expression was quantified using Image J. The images were shown at 20× and 40× magnifications for each experimental condition (scale bar=100 μm). (C) Cellular TG content measurement was performed in primary hepatocytes under PA and JB treatment. (D, E) Lipogenesis genes were measured using qRT-PCR in primary hepatocytes. (F, G) Expression of the lipogenic genes Acc and Scd1 determined by qRT-PCR in Hep3B cells. Relative mRNA expression levels were normalized to GAPDH levels. The data are expressed as means ± sem. *p<0.05, **p<0.01, and ***p<0.001.
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