Table 1 Primer sequences of the genes used for quantitative real-time polymerase chain reaction
| Gene | Forward (5’ to 3’) | Reverse (3’ to 5’) |
|---|---|---|
| Human | ||
| GAPDH | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC |
| SREBP-1c | ACAGTGACTTCCCTGGCCTAT | GCATGGACGGGTACATCTTCAA |
| ACC | TCGCTTTGGGGGAAATAAAGTG | ACCACCTACGGATAGACCGC |
| SCD1 | TGTGTCCCAGATGCTGTCATTA | CGTGGCAATGCGTTGTTTATG |
| CPT1α | TCCAGTTGGCTTATCGTGGTG | TCCAGAGTCCGATTGATTTTTGC |
| IL-6 | AAGCCAGAGCTGTGCAGATG | TGTCCTGCAGCCACTGGTTC |
| TNF-α | CTGCCTGCTGCACTTTGGAG | ACATGGGCTACAGGCTTGTCA |
| SOCS3 | CCTCAAGACCTTCAGCTCCA | TCACTGCGCTCCAGTAGAAG |
| MT-ND1 | ACCCCCGATTCCGCTACGACCAAC | GGTTTGAGGGGGAATGCTGGA |
| Mouse | ||
| GAPDH | GCCAAGCCTGCTTCTTACTC | TGAGGGCAATTCCAGCCTTA |
| SREBP-1c | ACACTTCTGGAGACATCGCA | CGGATGAGGTTCCAAAGCAG |
| ACC | CTCCCGATTCATAATTGGGTCTG | TCGACCTTGTTTTACTAGGTGC |
| SCD1 | TTCTTGCGATACACTCTGGTGC | CGGGATTGAATGTTCTTGTCGT |
| STAT5a | CAGATGCAAGTGTTGTATGGGC | GCTGGCTCTCGATCCACTG |
| CPT1α | CGGTTCAAGAATGGCATCATC | TCACACCCACCACCACGAT |
| IL-6 | ACAAAGCCAGAGTCCTTCAGAGAG | TTGGATGGTCTTGGTCCTTAGCC |
| TNF-α | CCAACGGCATGGATCTCAAA | CCCTTGAAGAGAACCTGGGA |
| SOCS3 | GCAGGAGAGCGGATTCTACT | TGGATGCGTAGGTTCTTGGT |
| MT-ND1 | CTAGCAGAAACAAACCGGGC | CCGGCTGCGTATTCTACGTT |
| 16s rRNA | CCGCAAGGGAAAGATGAAAGAC | TCGTTTGGTTTCGGGGTTTC |
| HK2 | GCCAGCCTCTCCTGATTTTAG | GGGAACACAAAAGACCTCTTCT |
GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SREBP-1c, Sterol regulatory element-binding transcription factor 1; ACCα, 1-Aminocyclopropane-1-Carboxylate; SCD1, Stearoyl-CoA desaturase-1; IL-6, Interleukin 6; TNF-α, Tumor necrosis factor-alpha; SOCS3, Suppressor of cytokine signaling 3; MT-ND1, Mitochondrially encoded NADH dehydrogenase 1; 16s rRNA, 16S ribosomal RNA; HK2, Hexokinase 2.