Fig. 3. Knockdown of eIF2S2 inhibits invasion and migration in cervical cancer cells. (A) SiHa cells that were stably transfected with either control or eIF2S2 shRNA (clone-3 and clone-4) underwent the anchorage-independent cell transformation assay. Data represent three independent experiments. (B) The average number of colonies in each group was calculated and the statistical analysis was conducted to measure the significance test. (C) SiHa and (D) HeLa cells were transiently transfected with control or eIF2S2 siRNA and cell migration assay was conducted using a 24-well transwell after 24 h of serum starvation. *p<0.001, compared to control siRNA. (E) SiHa and (F) HeLa cells were transfected with either control or eIF2S2 si-RNA for various duration. A wound healing assay was performed as described in the Materials and Methods section.
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