Fig. 2. Effect of eIF2S2 knockdown on viability and growth in vitro and in vivo. (A) SiHa and (B) HeLa cells were transfected with control or eIF2S2 siRNA for 24, 48, or 72 h. The survival fractions of cells were obtained by the water-soluble tetrazolium salts (WST) assay. (C) SiHa and (D) HeLa cells were transfected with control or eIF2S2 siRNA consecutively for 1 and 2 days and were left growing until 6 days to assess the long-term effect of eIF2S2 siRNA on cell proliferation. Cell proliferation was evaluated by trypan blue dye-excluding method. All experiments were conducted in triplicate. *p<0.001, compared to control siRNA. (E, F) Female BALB/c (nu/nu) mice were subcutaneously injected with SiHa-sh-control cells (n=3) or SiHa-sh-eIF2S2-clone-4 cells (n=6). Animals were sacrificed after 107 days of inoculation. *p<0.001, significantly different from the control group.
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