Fig. 7. Effect of β-Lap on levels of A1R mRNA and NF-kB p65 protein. The changes in (A) A1R mRNA levels in the cortex of ICR mice. Gene expression was normalized to the mRNA levels of β-actin. β-Lap (1 mg/kg, p.o.) was administered, and 1 h later, the mice were sacrificed. DPCPX (5 mg/kg, p.o.) was administered 15 min before administration of β-Lap. The changes in (B) p65 expression in the nucleus extracts. Western blot analysis of the expression level of p65 in the nucleus was normalized to Lamin B1. The changes in (C) p65 expression in the cytoplasm extracts. Western blot analysis of the expression level of p65 in the cytoplasm was normalized to β-actin. Data are expressed as mean ± SEM (n=3). Effect of NF-κB inhibitor on the changes in (D) sleep onset latency and (E) total sleep time. SN-50 (1 mg/kg, i.p.) was injected to mice 30 min before administration of β-Lap (1 mg/kg, p.o.). Pentobarbital (45 mg/kg, i.p.) was injected 30 min after administration of β-Lap. Data are expressed as mean ± SEM (n=5). *p<0.05 vs CTL; **p<0.01 vs CTL; #p<0.05 vs VEH; ##p<0.01 vs VEH. CTL, control; VEH, Vehicle; β-Lap, β-lapachone; DPCPX, 8-cyc lopentyl-1,3-dipropylxanthine; p.o., Per oral; i.p., Intraperitoneal.
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