Fig. 4. Effect of β-Lap on orexinergic neuronal activity in LH. β-Lap (1 mg/kg, p.o.) was administered 1 h before brain extraction. β-Lap was administered 15 min after administration of DPCPX (5 mg/kg, p.o.). (A) Photomicrographs of c-Fos expression (black arrows) at low magnification and high magnification representative of LH. (B) Quantitative analysis for c-Fos-positive cells. (C) Triple immunofluorescence image showing orexin-A (OrxA; green), c-Fos (red), and DAPI (blue), with white arrows showing c-Fos merging with the DAPI in LH. Data are expressed as means ± SEM (n=7-10). **p<0.01 vs CTL; ##p<0.01 vs VEH. CTL, control; VEH, Vehicle; β-Lap, β-lapachone; DPCPX, 8-cyc lopentyl-1,3-dipropylxanthine; p.o., Per oral.
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