Fig. 3. Effect of β-Lap on cholinergic neuronal activity in BF. β-Lap (1 mg/kg, p.o.) was administered 1 h before brain extraction. β-Lap was administered 15 min after administration of DPCPX (5 mg/kg, p.o.). (A) Photomicrographs of c-Fos expression (black arrows) at low magnification and high magnification representative of BF. (B) Quantitative analysis for c-Fos-positive cells. (C) Triple immunofluorescence image showing choline acetyltransferase (ChAT; green), c-Fos (red), and DAPI (blue), with white arrows showing c-Fos merging with the DAPI in BF. Data are expressed as means ± SEM (n=7-10). **p<0.01 vs CTL; ##p<0.01 vs VEH. CTL, control; VEH, Vehicle; β-Lap, β-lapachone; DPCPX, 8-cyc lopentyl-1,3-dipropylxanthine; p.o., per oral.
© Biomolecules & Therapeutics
