Fig. 2. The expression of VISTA in CML patients and cells. (A) Scatter plot showing the levels of
VSIR expression in CML patients (n=9) and healthy donors (n=9) in the GSE33075 dataset (
p<0.05); (B) Patterns of VSIR expression in 40 CML samples from the GSE77191 dataset across three phases (chronic, accelerated, and blast phases). (C) The
VSIR of CD34+ G1/S/G2/M (n=4) CML patients was greater than that of normal CD34+ G1/S/G2/M (n=8) CML patients based on the GSE24739 dataset (
p<0.05). (D) The cell line mRNA expression matrix of CML cells was obtained from the CCLE dataset (
https://portals.broadinstitute.org/ccle). The analysis was performed with the R v4.0.3 software package ggplot2 (v3.3.3). (E, F) Protein and mRNA levels of VISTA in CML cells (KBM5, KBM5-T315I, K562 and K562-R cells) determined via Western blotting and qRT‒PCR. (G) Immunofluorescence results showing the localization of VISTA (green) in both the cytoplasm and nucleus of CML cells (blue: DAPI, bar: 50 μm). Asterisks (*) indicate significance, ** indicates
p<0.01, * indicates
p<0.05, and NS indicates no significance.
