Fig. 5. Effect of pyronaridine on phorbol 12-myristate 13-acetate (PMA)-induced phosphorylation and translocation of NF-kB p65 in NCI-H292 cells. Nuclear protein extracts were prepared and subjected to western blotting using a phospho-specific p65 (Ser 536) antibody and an antibody against p65. p84 levels were analyzed as a loading control. Three independent experiments were performed, and representative data are shown (n=3). *Significantly different from that of the control (p<0.05). +Significantly different from that with PMA treatment alone (p<0.05). Cont: control, P: pyronaridine, concentration unit is μM.
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