Fig. 4. Effect of pyronaridine on phorbol 12-myristate 13-acetate (PMA)-induced IκBα phosphorylation and IκBα degradation in NCI-H292 cells. NCI-H292 cells were incubated with varying concentrations of pyronaridine for 24 h, and then treated with 50 ng/mL PMA for 30 min. Cytoplasmic extracts were fractionated and then subjected to western blot analysis using a phospho-specific IκBα (Ser 32/36) antibody or an antibody against IκBα. Equal protein loading was evaluated by β-actin levels. Three independent experiments were performed, and representative data are shown (n=3). *Significantly different from that of the control (p<0.05). +Significantly different from that with PMA treatment alone (p<0.05). Cont: control, P: pyronaridine, IκBα: inhibitory kappa Bα, concentration unit is μM.
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