Fig. 2. Inhibitory ability of ARO on LPS-induced NF-κB activation in BEAS-2B cells. (A) The expression levels of p-NF-κB, p-IκBα, MyD88 and TLR4 in whole cell lysates (WCL) detected using western blotting. BEAS-2B cells were maintained ARO (25–200 μM) for 1 h and incubated in the presence of LPS (1 μg/mL) for 1 h. Quantitative analysis of (B) p-p65, (C) p-IқB, (D) MyD88, and (E) TLR4 was performed using the ImageJ software (version 1.50e; National Institute of Health, MD, USA). Data are expressed as the mean ± SD (#p<0.01 for comparison with the control; *p<0.05 for comparison with the LPS group). ARO stands for aromadendrin.
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