Table 1 List of antibodies
Primary antibody | Company | Molecular weight | Dilution | Host | Secondary antibody |
---|---|---|---|---|---|
β-action (sc-47778) | Santa Cruz (Dallas, TX, USA) | 43 | 1:2000 | Mouse | Goat anti mouse-HRP |
CD68 (sc-20060) | Santa Cruz | 75-110 | 1:1000 | Mouse | Goat anti mouse-HRP |
COX-2 (sc-376861) | Santa Cruz | 70-72 | 1:1000 | Mouse | Goat anti mouse-HRP |
HO-1 (PA5-27338) | Invitrogen | 32 | 1:1000 | Rabbit | Goat anti rabbit-HRP |
IκB-α (MA5-15132) | Invitrogen | 36 | 1:1000 | Mouse | Goat anti mouse-HRP |
iNOS (ab136918) | Abcam (Cambridge, UK) | 130 | 1:1000 | Rabbit | Goat anti rabbit-HRP |
MyD88 (sc-74532) | Santa Cruz | 33 | 1:1000 | Mouse | Goat anti mouse-HRP |
NQO1 (N5288) | Sigma (St. Louis, MO, USA) | 28 | 1:1000 | Rabbit | Goat anti rabbit-HRP |
p65 (sc-8008) | Santa Cruz | 65 | 1:1000 | Mouse | Goat anti mouse-HRP |
p-IκBα (2859S) | Cell Signaling (Danvers, MA, USA) | 40 | 1:1000 | Rabbit | Goat anti rabbit-HRP |
p-p65 (3033S) | Cell Signaling | 65 | 1:1000 | Rabbit | Goat anti rabbit-HRP |
p-STAT3 (9145S) | Cell Signaling | 79-86 | 1:1000 | Rabbit | Goat anti rabbit-HRP |
STAT3 (12640S) | Cell Signaling | 79-86 | 1:1000 | Rabbit | Goat anti rabbit-HRP |
TLR4 (sc-293072) | Santa Cruz | 95-120 | 1:1000 | Mouse | Goat anti mouse-HRP |