Fig. 6. Effects of leptin on apoptosis, p16 expression, and modulation of AKT/p38MAPK pathways in hepatocyte specific IL-1R1 knockout mice liver. (A) Hepatocyte specific IL-1R1 knockout mice was generated using loxP/Cre system. Messenger RNA levels of the corresponding genes were meaured by PCR amplification as described in the methods. In left panel, lane-1: IL-1R1fl/fl mice, lane-2: heterozygous for IL-1R1, lane-3: WT for IL-1R1, lane-4: IL-1R1fl/fl mice. In right panel, lanes 1 and 2: Cre recombinase detected, lanes 3rd and 4th: no expression of Cre recombinase. (B, C) Expression levels of IL-1R1 in liver (B) and hepatocytes (C) were measured by western blot analysis. (D-I) Mice were adminstered with leptin (1 mg/kg) intraperitoneally twice a day for 15 days. After treatment, livers were prepared as indicated in the methods and expression levels of cleaved caspase-3 (D), cleaved cytokeratin-18 (E), Bax (F), Bcl-2 (G), p16 (H), phosphor-AKT (I), and phosphor-p38MAPK (J) were measured by western blot analysis. Representative images from three mice out of total five mice in each group is presented. Values are presented as mean ± SEM, n=5. * denotes p<0.05 in comparison to WT-control. # denotes p<0.05 in comparison to WT-leptin.
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