Table 2 Sequences of the primers used for PCR amplification
| Target gene | Forward sequence | Reverse sequence |
|---|---|---|
| p16 | 5'-CGGTATCTACTCTCCTCCGC-3' | 5'-GTTGCCAGAAGTGAAGCCAA-3' |
| p21 | 5'-AACTGGGGAGGGCTTTCTTT-3' | 5'-TGTCTTGTCTTCGCTGAGGT-3' |
| p27 | 5'-AACTAACCCGGGACTTGGAG-3' | 5'-GAGACCCAATTGAAGGCACC-3' |
| GAPDH | 5'-TGAACGGGGAAGCTCACTGG-3' | 5'-TCCACCACCCTGTTGCTGTA-3' |
| Cyclin D1 | 5'-CCCCAACAACTTCCTCTCCT-3' | 5'-AGCTTCTTCCTCCACTTCCC-3' |
| Cyclin E1 | 5'-AAGGAGGGTGCTACTTGACC-3' | 5'-TCAGCTGACAGTGGAGAAGG-3' |