Fig. 4. Effects of carnosine and ROL on HA secretion in NHEKs. NHEKs were cultured in 6-well plates. Upon reaching confluence, carnosine and ROL were treated. Cell culture supernatants were analyzed for HA secreted content by ELISA. The concentrations of HA induced by carnosine and ROL individually (A) and in combination (B) were quantified 48 h after the treatment. (C) The concentration dependent effectsof carnosine and ROL co-treatment on HA production were determined. (D) Pearson’s correlation coefficient (R2) between the HA levels and PGE2 levels were calculated. Values represent the mean expression ± standard deviation (SD) (n=3). *p≤0.05 vs. vehicle control; **p≤0.01 vs vehicle control and ##p≤0.01 vs. ROL 10 μM.
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