Fig. 2. Effects of carnosine and ROL on PGE2 production in NHEKs. (A) Structures of L-carnosine and ROL. NHEKs were cultured in 48-well plates. When NHEKs reached 100% confluence, UVB irradiation was applied at a dose of 20 mJ/cm2, and varying concentrations of carnosine and ROL were treated. (B) The quantification of PGE2 levels in cell culture supernatants was performed using ELISA. Aspirin (ASA, 300 μM) was used as a positive control drug. (C) The concentration dependency of carnosine and ROL co-treatment on PGE2 production was determined. Values represent the mean expression ± standard deviation (SD) (n=3). *p≤0.05 vs. UVB (+) vehicle control; **p≤0.01 vs. UVB (+) vehicle control; #p≤0.05 vs. ROL 10 μM; ##p≤0.01 vs. ROL 10 μM; Φp≤0.05 vs. ROL 3 μM; ΦΦp≤0.01 vs. ROL 3 μM; θp≤0.05 vs. ROL 1 μM and θθp≤0.01 vs. ROL 1 μM.
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