Fig. 5. Induction of ROS generation by domperidone in HCT116 cells. HCT116 cells were stimulated with domperidone (0, 5, 25, and 50 μM) for 6, 12, 24, and 48 h. The intracellular accumulation of ROS was identified by measuring the fluorescence of DCF-DA using FACS and fluorescence microscopy. (A, C) Histogram of DCF fluorescence. (B, D) DCF fluorescence microscopic images. (E) Graph of DCF fluorescence intensity. Data are shown as the mean ± standard deviation (SD), (n=3). *p<0.05.
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