Fig. 1. Disturbance of the antiapoptotic Bcl-2 protein by domperidone. (A) Immunoblot analysis of mitochondrial apoptosis-related proteins Bcl-2 and cytochrome C. (B) Immunoblot analysis of mitochondrial apoptosis-related proteins cytosolic Bcl-2 and cytochrome C in HCT116 cells. (C) Immunoblot analysis of mitochondrial apoptosis-related proteins Bcl-2 and cytochrome C in the mitochondrial fraction of HCT116 cells and COX IV was used as the loading control. (D) The levels of cleaved forms of caspase-3, -7, and -9 and PARP monitored by immunoblot. β-Actin was used as the loading control.
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