Fig. 8. Attenuation of the protective ability of mangiferin against H2O2-induced ROS production and apoptosis by ZnPP in ARPE-19 cells. Cells were treated with mangiferin (20 µM) and/or ZnPP (10 µM) for 1 h and then further treated with H2O2 (0.5 mM) for 24 h. (A, B) Representative flow cytometry results (A) and their average values (B) according to DCF-DA staining. (C, D) Representative histograms (C) and quantitative results (D) of flow cytometry analysis following double staining of annexin V/PI. (E) Cell viability was assessed using the MTT assay. All data are represented as mean SD from triplicate experiments (***p<0.001 compared with the control group. ###p<0.001 compared with the H2O2-treated group. $$$p<0.001 compared with the mangiferin and H2O2-treated group).
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