Fig. 7. Activation of the Nrf2/HO-1 signaling by mangiferin in H2O2-treated ARPE-19 cells. Cells exposed with or without mangiferin (20 µM) for 1 h were stimulated with H2O2 (0.5 mM) for 24 h. Resveratrol (25 µM) was treated alone for 24 h and was used as a positive regulator of Nrf2 activation. (A, B) Expression changes of proteins presented in each treatment group were analyzed by immunoblotting using total proteins (A) or cytosolic and nuclear fractions (B). (C) The activity of HO-1 in each treatment group was expressed as a relative value. All data are represented as mean SD from triplicate experiments (***p<0.001 compared with the control group. ###p<0.001 compared with the H2O2-treated group).
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