Fig. 6. Mangiferin abolished H2O2-induced production of mtROS and inactivation of MnSOD and PGx in ARPE-19 cells. Before treating the cells with H2O2 (0.5 mM) for 24 h, they were incubated in the presence or absence of mangiferin (20 µM) for 24 h. (A, B) Representative histograms of flow cytometry following MitoSOX staining (A) and the frequency of MitoSOX-positive cells. (C) Expression changes of MnSOD and PGx obtained through immunoblotting. (D, E) Changes in the enzymatic activity of MnSOD and PGx were obtained using commercially available assay kits. All data are represented as mean SD from triplicate experiments (***p<0.001 compared with the control group. #p<0.05, ##p<0.01 and ###p<0.001 compared with the H2O2-treated group).
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