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Fig. 3. Mangiferin suppressed H2O2-induced apoptosis in ARPE-19 cells. Before treating the cells with H2O2 (0.5 mM) for 24 h, they were incubated in the presence or absence of mangiferin (20 ┬ÁM) for 1 h. (A, B) Representative histograms (A) and quantitative results (B) of flow cytometry analysis by annexin V/PI staining. (C) Expression changes of the indicated proteins obtained through immunoblotting. (D) Differences in caspase-3 activity were presented in each treatment group. All data are represented as mean SD from triplicate experiments (***p<0.001 compared with the control group. ###p<0.001 compared with the H2O2-treated group.)
Biomolecules & Therapeutics 2024;32:329~340 https://doi.org/10.4062/biomolther.2023.175
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