Biomolecules & Therapeutics : eISSN 2005-4483 / pISSN 1976-9148

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Fig. 5. Effect of salmon nasal cartilage proteoglycan (SPG) on collagen degradation in UVB-irradiated in vitro and in vivo models. (A) Masson’s trichrome staining of mouse skin tissue was performed on the last day of the experiment (×40; bar size 100 μm). (B) Immunohistological changes in procollagen type 1 in the skin of SKH-1 mice exposed to UVB irradiation for eight weeks (×40; bar size 100 μm). (C, D) ELISA kit and western blot analysis for measuring the expression of MMP-1 and elastin in NHDF cells. ELISA was performed to quantify MMP-1 in the supernatant of the cell culture medium. All protein levels were normalized to those of α-tubulin. The values are presented as mean ± SEM (n=3-4). **p<0.01 and ***p<0.001 vs. Ctrl, #p<0.05, ##p<0.01, and ###p<0.001 vs. NC.
Biomolecules & Therapeutics 2024;32:249~260
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