Fig. 4. Effect of salmon nasal cartilage proteoglycan (SPG) on ceramide content in UVB-irradiated mouse model, and the mRNA level of human glucosylceramide synthase (hGCS) and hyaluronan content in UVB-irradiated HaCaT cells. (A) HPLC analysis of the ceramide content in mouse epidermis. (B) mRNA levels of hGCS in HaCaT cells treated with PG for 1–12 h. All mRNA levels were normalized to those of GAPDH. (C) Effect of SPG on cell viability and hyaluronan secretion in UVB-irradiated HaCaT cells. ELISA was performed to quantify hyaluronan in the supernatant of the cell culture medium. The values are presented as mean ± SEM (n=4-8). *p<0.05, **p<0.01, and ***p<0.001 vs. Ctrl, #p<0.05, ##p<0.01, and ###p<0.001 vs. NC.
© Biomolecules & Therapeutics
