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Fig. 6. DMK inhibits AP-1 transactivation in HaCaT cells. (A) Diagram demonstrating the generation of pGreenFire-HaCaT-AP-1-GFP-luciferase cells. (B) Established HaCaT-AP-1-GFP-luciferase cells (1×106 cells) were exposed to DMK for 24 h at various concentrations, and AP-1-dependent luciferase activity was measured by the luciferase assay (n=3). (C) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the protein expression of c-JUN and c-FOS was measured by Western blot analysis. (D) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the mRNA levels of c-Fos and c-Jun were measured by real-time RT-PCR (n=4). **p<0.01 and ***p<0.001 vs Control (DMSO).
Biomolecules & Therapeutics 2024;32:240~248 https://doi.org/10.4062/biomolther.2023.131
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