Fig. 5. The inhibition of c-Jun and selected MMPs by DMK is attributable to the attenuation of the IL1α/β-IL1R-ERK1/2-AP1 axis in HaCaT cells. (A) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the mRNA levels of IL-1α and IL-1β were measured by real-time RT-PCR analysis (n=4). (B) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the mRNA levels of IL-1R were measured by real-time RT-PCR analysis (n=4). (C) HaCaT cells (2×106 cells) were exposed to DMK at various times, and the level of ERK1/2 phosphorylation was measured by Western blot analysis. (D) The mRNA levels of c-Jun were measured in HaCaT cells by real-time RT-PCR after IL-1R was knocked down (n=4). (E) The mRNA levels of MMP-1, MMP-9, MMP-10, and MMP-12 were measured in HaCaT cells by real-time RT-PCR after IL-1R was knocked down (n=4). *p<0.05, **p<0.01, and ***p<0.001 vs Control (DMSO).
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